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Related post: TITLE OF PROJECT (80 characters or less. Title must tit on one line between the borders) Serotype Analysis and Characterization of Rotaviruses from Malaysia PRINCIPAL INVESTIGATOR (Ust other professional personnel below the Principal Investigator.) (Name, title, laboratory, and institute affiliation) PI: Nassar Rasool, Ph.D. Guest Researcher LID, NIAID Others: Catapres Tts 2 Albert Z. Kapikian, M.D. Head, Epid. Section LID, NIAID Kim Y. Green, Ph.D. Senior Staff Fellow LID, NIAID COOPERATING UNITS (if any) LAB/BRANCH Laboratory of Infectious Diseases SECTION Epidemiology Section INSTITUTE Catapres Tts AND LOCATION NIAID, NTH, Bethesda, MP 20892 TOTAL MAN-YEARS: 0.1 PROFESSIONAL: 0.1 CHECK APPROPRIATE BOX(ES) □ (a) Human subjects □ (b) Human tissues E (c) Neither □ (a1) Minors D (a2) Interviews SUMMARY OF WORK (Use standard unreduced type. Do not exceed the space provided.) A total of 341 stool specimens known to be positive Catapres 0.1 Mg for group A rotaviruses by RNA electrophoresis had been obtained from various locations in Malaysia from children below the age of five years who had gastroenteritis. These stool specimens which were collected from 7 of the 12 years spanning 1977-1988 were brought Catapres Clonidine to the NIH for determination of the serotypes circulating in Malaysia and for further characterization by molecular biologic techniques. Ten percent suspensions of 294 specimens were Catapres 100 tested for antigen by a pre-post confirmatory ELISA. Forty-seven specimens appeared to be too dilute or were limited in quantity and thus, were not tested by this system, but rather were passaged into primary Buy Catapres African green monkey kidney (AGMK) cells using the method of Ward et al. (1984). Of the 285 specimens that were ELISA positive, 176 could be serotyped: 71% belonged to serotype 4, 15% to serotype 1, 4% to serotype 3 and 3% to serotype 2. In addition, 6% displayed ambiguous serotype specificities, reacting to more than one monoclonal antibody, and need to be investigated further. 11-75 PHS 6040 (Rev. 1/84) CPO »I4-»I« PROJECT NUMBER DEPARTMENT OF HEALTH AND HUMAN SERVICES - PUBLIC HEALTH SERVICE NOTICE OF INTRAMURAL RESEARCH PROJECT j Z01 AI 00577-01 LID PERIOD COVERED October 1, 1989 to September 30, 1990 TITLE OF PROJECT (80 characters or less Title must lit on one line between the borders ) Molecular Characterization of the VP4 Gene of Strain 69M, a New Human Rotavirus Serotype PRINCIPAL INVESTIGATOR (Ust other professional personnel below the Pnncipal Investigator ) (Name, title, laboratory, and institute affiliation) PI: Yuan Qian, M.D. Visiting Associate LID, NIAID Others: Kim Green, Ph.D. Senior Staff Fellow LID, NIAID COOPERATING UNITS (if any) LAB/BRANCH Laboratory of Infectious Diseases SECTION Epidemiology Section INSTITUTE AND LOCATION NIAID, NIH, Bethesda, MP 20892 TOTAL MAN-YEARS: 0.2 PROFESSIONAL: 0.2 CHECK APPROPRIATE BOX(ES) □ (a) Human subjects Catapres Tts Patch □ (b) Human tissues 63 Buy Catapres Online (c) Neither □ (a1) Minors □ (a2) Interviews SUMMARY OF WORK (Use standard unreduced type. Do not exceed the space provided ) Human group A rotavirus, strain 69M, isolated from an ill child in Indonesia, has been described as a Catapres 0.2 Mg new serotype (serotype 8). Previous sequence analyses in our laboratory of the gene encoding the outer capsid protein VP7 confirmed that 69M is distinct from established human rotavirus serotypes 1, 2, 3 and Catapres Tts 3 4. In order to assess whether, VP4 (encoded by gene 4), the other major outer capsid neutralization protein of 69M is also distinct, the complete nucleotide sequence of the gene 4 was determined. The sequence of the 69M VP4 gene was compared with available VP4 gene sequences from human and animal rotaviruses. Catapres Patches In addition, Northern blot hybridization was performed to examine the genetic relatedness of the 69M VP4 gene with other group A rotaviruses using 32 P-labeled cloned 69M VP4 gene DNA or 69M ssRNA transcript probes. These genetic studies as well as neutralization confirmed that the fourth gene (encoding VP4) of strain 69M is different from any of the other corresponding genes described thus far. Of interest, comparative analyses of the deduced Catapres Tablets amino acid sequences suggest that the Catapres Patch Tts VP4 of 69M is more closely related to animal rotaviruses than to human rotaviruses. 11-76 PHS 6040 (Rev MM) GPO M*»ll PROJECT NUMBER DEPARTMENT OF HEALTH AND HUMAN SERVICES - Clonidine Catapres PUBLIC HEALTH SERVICE NOTICE OF INTRAMURAL RESEARCH PROJECT ' Z01 AI 00582-01 LID PERIOD COVERED October 1, 1989 to September 30, 1990 TITLE OF PROJECT (80 characters or less. Title must lit on one line between the borders.) Expression and Recovery of Recombinant Rotavirus VP4 from Insect Cell Cultures PRINCIPAL INVESTIGATOR (Ust other prolessional personnel below the Pnncipal Investigator ) /Name, title, laboratory, and institute affiliation) PI: Mario Gorziglia, Ph.D. Visiting Associate LID, NIAID Others: Luis G. Juarbe-Osorio Guest Researcher LID, NIAID COOPERATING UNITS (if any) LAB/BRANCH Laboratory of Infectious Diseases SECTION Epidemiology Section INSTITUTE AND LOCATION NIAID, NTH, Bethesda, MD 20892
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